Wed, Jun 25th - 2:43AM

The Microsoft 70-291 Exam Practice Engine

The Microsoft 2003 Server Exam, 70-291, is one of the core exams of the MCSE and MCSA certification tracks. The exam tests a candidate's knowledge of Windows 2003 networking environments and how to implement a successful Windows Server 2003 network in either existing Windows environments or on totally new networks. The exam features simulation and analysis questions that require the candidate to have a thorough working knowledge of the Windows Server 2003 operating system and the way that a Windows 2003 network operates.

The Microsoft Certified Systems Administrator (MCSA) on Windows Server 2003 credential is intended for IT professionals who work in the typically complex computing environment of medium to large companies. An MCSA candidate should have 6 to 12 months of experience administering client and network operating systems in environments that have the following characteristics:

• 250 to 5,000 or more users
• Three or more physical locations
• Three or more domain controllers
• Network services and resources such as messaging, database, file and print, proxy server, firewall, Internet, intranet, remote access, and client computer management
• Connectivity requirements such as connecting branch offices and individual users in remote locations to the corporate network and connecting corporate networks to the Internet

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Fri, Jun 6th - 4:00AM

Cell Culture Guidelines

Cell culture is an invaluable tool for investigators in numerous fields. It facilitates analysis of biological properties and processes that are not readily accessible at the level of the intact organism. Successful maintenance of cells in culture, whether primary or immortalized, requires knowledge and practice of a few essential techniques.

The purpose of these words are guidelines for cell culture.

At first,you should make sure several questions below

Several factors should be considered before a cell line is put into culture

• Are the cells coming from established, well-defined lines?
• Are the nutrient and CO2 requirements known?
• Are the growth patterns known?
• Is the species known?
• Has mycoplasma testing been done?

After consideration of all the above, thoughtful planning should be given to other factors

• Will the cells be cultured in cell culture dishes, plates, flasks, bioreactors, etc.
• Who will do the tissue culture?
• Who will prepare, test and maintain stocks of media, regents and supplies?
• What volume/number of cells will be required?

The following are general guidelines for culturing cells

If hybridoma cells are received in a cell culture flask (or cell culture dish), information regarding the optimal type of transfer should be available. Once cells can be maintained in one type of culturing vessel, it should be easy to adapt them to other cultureware.

If tumor cells are received it is necessary to know if they are of fibrosarcoma or lymphoma origin. Fibrosarcoma cells will generally be adherent to plastic and require the use of trypsin or EDTA to remove them. Tumor cells also usually require less fetal calf serum in their media.

If cells are received frozen, in an ampule or cryovial, they should be washed free of DMSO prior to culturing.

Establish a routine for the passage/transfer of the cells based on the rate at which they form a monolayer. For example, culture the cells in 2 wells of a 24-well plate. On Monday, cell should be resuspended gently with a pasteur pipet or 1mL pipet and enough cells transferred, dropwise, into duplicate wells of fresh, warmed media so that 4 days later, a monolayer is again observed and the transfer is repeated. It may be useful to determine the cell concentration at various days in culture in order to make projections and appropriate expansions.

If cells require the addition of growth factors, dilutions must be made carefully and good tissue culture technique maintained.

Some cells can easily be adapted to various kinds of media. Adaptation should take place overtime, with adequate post-adaptation analysis of proliferation, secretion, function, etc. During the period of adaptation the original culture should be maintained

Successful culturing of cells requires frequent monitoring of growth, media color, contamination, etc. The greater the familiarity with a cell line, the greater the expectation for a problem-free culture.

You should be careful when using the cell culture dishes(35mm Cell Culture Dishes, 60mm Cell Culture Dishes, 100mm Cell Culture Dishes).

Thu, Jun 5th - 2:18AM

Silver Jewelry Wear Rules

Silver jewelry is a special thing in your daily life. You should find the best way to wear it. Maybe you are very puzzled how to choose a silver jewelry for your cloth. Now there are some rules can help you.

1. Professional clothes should come together with simple\ fine style silver necklace.

2. Leisure clothes should come together with special\ exaggerate style silver necklace.

3. Lovely clothes can come toether with silver bracelet with a little bell. It will let you very happy and lovely.

4. Silver jewelry with colorful man-made stone should go together with young girl.

5. Tibet silver jewelry must not go together with gold\golden jewelry. But different style silver jewelry can wear at the same time.

6. Tibet silver jewelry must not to together with professional cloth. It will let others think you are not professional person.

7. When you attend party or wear the gorgeous dress, you should come together with silver jewelry with diamond or other gem.

8. Silver jewelry will be oxidized and some part will turn to black. Please don't worry about it will affect you image. It looks like retro style. It's another sense of silver jewelry.

9. Silver jewelry belong to fashion things. Please prepare more styles in order to change to wear.

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Wed, Jun 4th - 2:15AM

The Meaning Of Rings And Fingers

Rings are circles. In all cultures the circle is a symbol of unity and eternity, encompassing all. In early cultures, rings were considered magical or sacred through their association with the sun, moon and gods, thus giving protection to the wearer by binding one with power and energy.

Some of the magical mythology of ancient times still influences how and why we wear rings. Kings, priests and healers wore rings on the index finger because that finger was thought to be especially powerful. Medicines were applied to the body with the index finger to give speed and power to their effect. Even today, wearers of healing gemstones wear the rings on the index finger to take the best advantage of the curing energies.

The strongest magical tradition that still holds fast today is the one about the third or ring finger. The ring finger was thought to contain a nerve that went directly to the heart. Wearing a wedding or engagement ring on the ring finger still symbolizes the binding commitment and union of hearts. The Meaning of Fingers When Wearing Rings:

Right Hand vs. Left Hand

In the dual nature of man, the right and left hands are part of his whole being yet represent his twofold conscious and subconscious mind. The left hand (the subconscious) is a reflection of his deeply buried instinct, beliefs and attitudes. The right hand (the conscious) reflects his logic and his awareness. Wearing rings on your left hand could symbolize your unknowing wish to strengthen the powers associated with the finger that wears the ring. Rings on the right hand reflect your desire to control your life and actions.

The Thumb: Will Power

The thumb represents the self. Set apart from other fingers but working in unison with them to give strength to the hand that the others cannot, the thumb symbolized our self in our world. It is associated with logic and will power which govern our actions. Rings on the thumb symbolize freedom and independence of thought and action.

The Index Finger: Authority & Ambition

This finger reflects our need to enhance our ego. The forefinger, throughout history, has represented authority and power. It is associated with leadership and ambition. Kings, priests and healers wore rings on the index finger because that finger was thought to be especially powerful. Nowadays, people wear rings on the index finger as a show of assertiveness.

The Middle Finger: Identity & Materialism

The middle finger is associated with our role in life. Being in the center of the hand, it symbolizes personal identity and those things that are at central to our world. Those wearing rings on the middle fingers are well balanced yet wish to be at the center of attention.

The Ring Finger: Emotion & Creativity

The third finger is associated with affections because it is the one with a direct connection to the heart. It also represents our creativity and artistic self. A wedding or engagement ring on this finger proclaims to the world that the wearer emotions and creative goals are committed to the giver of the ring. If the ring is acquired by the wearer instead of a mate, it symbolizes self worth and a commitment to one personal skills and talents.

Little Finger: Relationships

The little finger is at the opposite end from the thumb. While the thumb symbolizes the inner self, the little finger is associated with others outside of ourselves. It sits on the outside of our hands, facing the world. It is the relationship finger, expressing our attitudes towards other people, sex, and the material world. Rings on the little finger represent confidence and independence in personal and business relationships. Rings here can also express a person attitude about sexuality and how appealing they consider themselves to be.

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Tue, Jun 3rd - 1:55AM

How To Choose The Right Bracelet

In this article we explain how to choose the right bracelet. Your bracelet could be made in silver or gold, it could be a simple bracelet or be studded with stones and diamonds. Make sure that you know how to evaluate a good bracelet.

Step One

Basic Design: The first thing that you should consider is the basic design of the bracelet. The design should be streamlined without sharp or pointed edges. Sharp edges can not only injure delicate skin, but also get bent or dented easily. The type of mounting used for the diamonds and gemstones in your bracelet will depend on the design. Prongs used to mount gems need to be sturdy and strong. If the gemstones in your bracelet are mounted with thin needle-like prongs, the prongs can get caught in pockets and other places. This causes the prongs to gradually open up, this ofcourse loosens the gemstones which can then drop off. The design dimensions for your bracelet should take the metal weight into consideration. A wide width with low gold or silver weight, will mean that the metal is too thin. This can cause your bracelet to lose shape and get damaged.

Step Two

Metal Weight: The metal weight of the bracelet is another important thing to be checked. Good mounting of the gemstones or diamonds in your bracelet will need sufficient gold or silver weight. There can be no standard weight suitable for all bracelets, much will depend on the design, the number of gems and diamonds, the length and width of the bracelet etc. Looks can be deceptive so pay special attention to the weight of your bracelet. In general you can be sure that, ladies bracelets with a metal weight that is less than 10 grams will not be durable and sturdy. Your jeweller might tell you that, healthy metal weight will make your bracelet appear clumsy and bulky. Stop believing this nonsense, the truth is that the design needs to ensure that the bracelet does not appear bulky. The design for your bracelet should have good metal thickness, this will not be visible from the front of the bracelet. Compromising on the gold or silver weight of your bracelet would also mean that, the links that connect the segments of the bracelet will be weak. This can cause the bracelet to snap, it could then easily fall off your wrist and get lost. So remember that, good metal weight is essential for gold or silver bracelets.

Step Three

Gemstone Options:You should also be given a wide range of gemstone options to select for your bracelet. Many jewellers use whatever few gemstones they have and put a bracelet together. This does not work to your advantage as you are restricted in your choice. You could choose the gemstones in your bracelet based on color, type, birthstone or traditional beliefs. For example, a mother's bracelet could include the birthstone of the mother and all her children. Such family birthstone bracelets can be made only if a wide choice of gems is available. There is ofcourse the issue of budget that you have at your disposal. You might see a gorgeous sapphire bracelet but, the sapphires could drive the bracelet out of your budget. You should be allowed to choose the same bracelet with cheaper gems like, blue topaz, iolite or probably cheaper sapphires. A jeweller with limited access to gems and production facilities will restrict your choice too. There is also the issue of conditions in which your bracelet would be worn. For example, an emerald bracelet needs special care when wearing and storing, this is because of the nature of emeralds. You might pefer the same bracelet design with green tourmaline instead of emeralds. Your jeweller will need to satisfy your requirements and not the other way round.

Step Four

Choose The Metal: The metal for your bracelet will be a component of the price tag, a gold bracelet would add a substantial amount to the price tag. You should therefore have the option to choose gold or silver for your bracelet. Most jewellers are very eager to sell gold bracelets, but will either ignore you are provide you with second grade service if you talk about a silver bracelet. This is sad but true, so look for a jeweller who is willing to make a good quality silver bracelet for you if that is what you want. It is often said and rightly that, a good quality silver bracelet is better than a delicate and light weight gold bracelet. When you choose your silver bracelet remember that, no compromises regarding gemstone choice or craftsmanship need be made. For gold bracelets, you should have the option to select a 14k or 18k bracelet and also choose the gold color. If you are looking for a white gold bracelet, there is no reason why you should be happy with a yellow gold aquamarine bracelet. Jewellers make gold bracelets with low weight, this is done to allow lower price tags. They really don't care what problems these light weight junk will cause you after the payment has been made.

Step Five

Good Craftsmanship: Insist on good craftsmanship for your bracelet, this should be the case for gold or silver bracelets. Bad craftsmanship could also mean loose gemstones and badly connected links. It is true that mass produced bracelets cannot be given individual attention. This is the reason why a custom bracelet could be best for you. Good craftsmanship is essential for ladies and men's bracelets, make sure that your bracelet is well crafted. Good craftsmanship provides more than just good appearance for your bracelet, this will clearly work to your advantage in the longterm.

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